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human ace2 elisa kit picokine tm ; sku ek0997  (Boster Bio)


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    Boster Bio human ace2 elisa kit picokine tm ; sku ek0997
    Human Ace2 Elisa Kit Picokine Tm ; Sku Ek0997, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+elisa+kit+picokine/rabbit+monoclonal+anti+ace2+antibody/pmc08452093-194-19-21
    Average 90 stars, based on 1 article reviews
    human ace2 elisa kit picokine tm ; sku ek0997 - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2.HLA-DR4.Rag1KO.IL-2RγcKO.NOD) for COVID-19
    Article Snippet: ing to the manufacturer’s instructions and concentrated to 52 μl each. The amounts of hACE2 protein in these immunoprecipitates were quantified using the highly sensitive hACE2 ELISA kit PicoKine TM (Boster Biological Technology, cat#EK0997) per the manufacturer’s protocol. The recombinant hACE2 protein was serially diluted in the provided sample buffer. The human lung sample was diluted 1:100, and



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    a. Positive control (+) = human lung mRNA. Negative control (−) = primers alone. upper row , PCR amplicons of <t>hACE2</t> from the lungs of 4 HIS-DRAGA mice (2 females and 2 males) and 2 non-HIS-reconstituted DRAGA mice (1 female and 1 male), amplified using hACE2-specific primers. Lower row, PCR amplicons of the same lung samples in the upper row, amplified using mACE2-specific primers. b. hACE2 protein expression on alveolar epithelia as indicated by binding of the S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC in lung sections from a representative HIS-DRAGA female mouse and two HIS-DRAGA male mice. Upper panels, merged images of S1(RBD)-mFc 2a binding (green) and nuclei (blue, DAPI), and an enlargement of the hACE2 + alveolar epithelia. Lower panels , binding of S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC and an enlargement of the hACE2 + alveolar epithelia. c. Same staining protocol as in a for lung sections from two non-HIS reconstituted DRAGA mice showing very weak binding (female mouse) and no detectable binding (male mouse) of S1(RBD)-mFc 2a protein, indicating siginificantly weaker avidity of the S1 viral protein for the murine than the human <t>ACE2</t> receptor. d. lung section from a representative HIS-DRAGA female mouse showing hTMPRSS2 co-localization with hACE2 as revealed by S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC (green) and anti-human TMPRSS2-PE (red) on the epithelial wall of a bronchiole ( b ) and the endothelial wall of a pulmonary arteriole ( a ). A section of the bronchiole image is also shown enlarged 200X.
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    a. Positive control (+) = human lung mRNA. Negative control (−) = primers alone. upper row , PCR amplicons of <t>hACE2</t> from the lungs of 4 HIS-DRAGA mice (2 females and 2 males) and 2 non-HIS-reconstituted DRAGA mice (1 female and 1 male), amplified using hACE2-specific primers. Lower row, PCR amplicons of the same lung samples in the upper row, amplified using mACE2-specific primers. b. hACE2 protein expression on alveolar epithelia as indicated by binding of the S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC in lung sections from a representative HIS-DRAGA female mouse and two HIS-DRAGA male mice. Upper panels, merged images of S1(RBD)-mFc 2a binding (green) and nuclei (blue, DAPI), and an enlargement of the hACE2 + alveolar epithelia. Lower panels , binding of S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC and an enlargement of the hACE2 + alveolar epithelia. c. Same staining protocol as in a for lung sections from two non-HIS reconstituted DRAGA mice showing very weak binding (female mouse) and no detectable binding (male mouse) of S1(RBD)-mFc 2a protein, indicating siginificantly weaker avidity of the S1 viral protein for the murine than the human <t>ACE2</t> receptor. d. lung section from a representative HIS-DRAGA female mouse showing hTMPRSS2 co-localization with hACE2 as revealed by S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC (green) and anti-human TMPRSS2-PE (red) on the epithelial wall of a bronchiole ( b ) and the endothelial wall of a pulmonary arteriole ( a ). A section of the bronchiole image is also shown enlarged 200X.
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    a. Positive control (+) = human lung mRNA. Negative control (−) = primers alone. upper row , PCR amplicons of hACE2 from the lungs of 4 HIS-DRAGA mice (2 females and 2 males) and 2 non-HIS-reconstituted DRAGA mice (1 female and 1 male), amplified using hACE2-specific primers. Lower row, PCR amplicons of the same lung samples in the upper row, amplified using mACE2-specific primers. b. hACE2 protein expression on alveolar epithelia as indicated by binding of the S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC in lung sections from a representative HIS-DRAGA female mouse and two HIS-DRAGA male mice. Upper panels, merged images of S1(RBD)-mFc 2a binding (green) and nuclei (blue, DAPI), and an enlargement of the hACE2 + alveolar epithelia. Lower panels , binding of S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC and an enlargement of the hACE2 + alveolar epithelia. c. Same staining protocol as in a for lung sections from two non-HIS reconstituted DRAGA mice showing very weak binding (female mouse) and no detectable binding (male mouse) of S1(RBD)-mFc 2a protein, indicating siginificantly weaker avidity of the S1 viral protein for the murine than the human ACE2 receptor. d. lung section from a representative HIS-DRAGA female mouse showing hTMPRSS2 co-localization with hACE2 as revealed by S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC (green) and anti-human TMPRSS2-PE (red) on the epithelial wall of a bronchiole ( b ) and the endothelial wall of a pulmonary arteriole ( a ). A section of the bronchiole image is also shown enlarged 200X.

    Journal: bioRxiv

    Article Title: A Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO.IL-2Rγc KO. NOD) for COVID-19

    doi: 10.1101/2020.08.19.251249

    Figure Lengend Snippet: a. Positive control (+) = human lung mRNA. Negative control (−) = primers alone. upper row , PCR amplicons of hACE2 from the lungs of 4 HIS-DRAGA mice (2 females and 2 males) and 2 non-HIS-reconstituted DRAGA mice (1 female and 1 male), amplified using hACE2-specific primers. Lower row, PCR amplicons of the same lung samples in the upper row, amplified using mACE2-specific primers. b. hACE2 protein expression on alveolar epithelia as indicated by binding of the S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC in lung sections from a representative HIS-DRAGA female mouse and two HIS-DRAGA male mice. Upper panels, merged images of S1(RBD)-mFc 2a binding (green) and nuclei (blue, DAPI), and an enlargement of the hACE2 + alveolar epithelia. Lower panels , binding of S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC and an enlargement of the hACE2 + alveolar epithelia. c. Same staining protocol as in a for lung sections from two non-HIS reconstituted DRAGA mice showing very weak binding (female mouse) and no detectable binding (male mouse) of S1(RBD)-mFc 2a protein, indicating siginificantly weaker avidity of the S1 viral protein for the murine than the human ACE2 receptor. d. lung section from a representative HIS-DRAGA female mouse showing hTMPRSS2 co-localization with hACE2 as revealed by S1(RBD)-mFc 2a protein + goat anti-mouse IgG-FITC (green) and anti-human TMPRSS2-PE (red) on the epithelial wall of a bronchiole ( b ) and the endothelial wall of a pulmonary arteriole ( a ). A section of the bronchiole image is also shown enlarged 200X.

    Article Snippet: The amounts of hACE2 protein in these immunoprecipitates were then quantified using the highly sensitive human ACE2 ELISA kit PicoKine TM (Boster Biological Technology, Pleasanton, CA) per the manufacturer’s protocol.

    Techniques: Positive Control, Negative Control, Amplification, Expressing, Binding Assay, Staining